flow cytometer - An Overview
flow cytometer - An Overview
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At that time, the early indications have been dismissed as debris, but the retrospective analysis shown shared traits Along with the problematic cells in society. Tracing the an infection aided the lab build further laboratory procedures for screening and protection of assay-vital cell strains.
As samples are acquired to the Attune CytPix Flow Cytometer, the significant-velocity brightfield digital camera captures and merchants visuals of detected occasions in a charge as many as six,000 pictures for every next, depending upon the flow fee and picture size. For increased overall flexibility, Attune Cytometric Software package lets you modify the impression capture frequency as wanted and choose a specific gate for image seize, if needed.
(B) RAW 264.7 macrophages tend to be annotated inaccurately with regard to particle count because of their complexity and noncircular borders. The quantity of singlets accessible for Investigation improves when the person trains and employs a more accurate product.
Explore new insights into your experimental details by visualizing populations applying merged fluorescence and morphology info. Our software program interface wizards permit downsampling, concatenation (merged documents) and dimensionality equipment that happen to be designed in the application for an intuitive, streamlined details Evaluation knowledge.
*Pricing is indicative only and is also issue to change. Make sure you Get hold of your Agilent agent with any questions. The information set forth herein just isn't a proposal or a commitment, both equally of that happen to be subject to credit score approval, conditions and terms, and availability with Agilent. Agilent Fiscal Alternatives is available in picked countries.
In this article, a highly skilled consumer has gated singlets confidently. Soon after assessing the manual singlet gate, the CytPix picture-derived parameter ParticleCount reveals this gate contains over four% aggregates.
Pair your Attune flow cytometer using an autosampler for enhanced workflow performance, and incorporate robotic integration for optimum throughput.
Reliable image quality even at high throughput Acoustic focusing and also a significant-velocity digital camera Mix to image these T cells consistently at low or large flow fees. Effortlessly regulate concentrate and digicam settings to meet experimental needs.
Early signs of this contamination experienced originally been dismissed as debris. Backgating to find new populations. Backgating imaged cells about the Attune CytPix Flow Cytometer also allows you to use morphological attributes to find out attention-grabbing subpopulations that will not be evident from flow cytometry info on your own.
Buyers can perspective occasion pictures in actual-time to confirm sample high quality, optimize protocols, and record the morphology of every mobile population recognized for Examination. Imaging seize also Gains from acoustic focusing, which helps to placement the cells making sure that a sharp, centered impression is obtained.
Sound waves allow precise cell alignment, resulting in Excellent information excellent and ~10X speedier sample flow charges.
Application Be aware: Automatic image analysis lessens consumer-to-consumer variability in flow cytometry gating approaches
Leverage the power of Sophisticated technological know-how and innovation to speed up your discoveries. Have faith in from the Attune Flow Cytometer spouse and children to provide the dependability, pace, and precision that set your perform apart.
Flexible payment strategy from Agilent Economic Remedies assist you to receive the crucial instrumentation you need for quick job, or to increase your lab.
Gating on these extended parameters means that you can rapidly and correctly recognize populations of interest and make sure gating technique with little or no manual overview. The Attune computer software provides usage of point out-of-the-artwork device Discovering Assessment tools inside an expertly built read more person interface, rendering it extremely simple to use.
As the mobile line had previously been analyzed within the Attune CytPix Flow Cytometer, the researchers went again to the images and have been capable of document the microbial infection no less than five days earlier.
Agilent Price Guarantee guarantees you a minimum of 10 years of instrument use from your date you buy. Usually, We're going to credit history you With all the residual value of the program toward an upgraded model.
What appeared to be a homogeneous inhabitants is definitely much more diverse—an Perception that should be considered when interpreting success.